Prolonged survival of Neisseria gonorrhoeae in a new liquid medium.

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Prolonged survival of Neisseria gonorrhoeae in a new liquid medium.

Despite substantial improvements in solid culture media for gonococci and the development of liquid media for the short-term growth of the organism, we know of no study of the long-term survival of Neisseria gonorrhoeae in liquid medium. A new liquid medium for N. gonorrhoeae formulated in this laboratory is simple, efficient, and promising. It will support growth and sustain inoculated colony ...

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Dense cultures of Neisseria gonorrhoeae in liquid medium.

Cultivation of Neisseria gonorrhoeae was effected in a conical glass culture vessel surrounded by a constant-temperature water jacket, and with facilities for stirring, aeration, and pH measurement and control. With the use of an aerated peptone-based medium, containing polypropylene glycol to prevent foam build-up, the yields obtained over the pH range from 5.8 to 7.4 were determined. The grea...

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Studies on Neisseria gonorrhoeae cultured in liquid medium.

Experiments were carried out to determine the minimum amount of inoculum of gonococci that is needed to produce growth in ANM liquid medium. This was found to be less than had previously been thought. Minimum inocula were used to study penicillin sensitivity in liquid medium. This work showed that growth occurred at higher concentrations of penicillin with larger inocula. Evidence was also obta...

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New selective medium for the isolation of Neisseria gonorrhoeae.

GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens. With stock cultures, vancomycin-resistant Staphylococcus epidermidis that grew on modified Thayer-Martin medium (MTM) was inhibited on the new medium. Also, vancomycin-susceptible strains of N. gonorrhoeae were mu...

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Further studies with a selective medium for cultivating Neisseria gonorrhoeae.

A culture medium that is selective for the gonococcus and the meningococcus has been described recently (Thayer and Martin, 1964). The medium with Polymyxin and Ristocetin added permits isolation of the gonococcus from sites highly contaminated with other bacteria. Overgrowth of gonococcal colonies by the commensal bacterial flora present in rectal, vaginal, and cervical specimens is almost com...

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ژورنال

عنوان ژورنال: Sexually Transmitted Infections

سال: 1976

ISSN: 1368-4973

DOI: 10.1136/sti.52.6.381